Category " Artigos Publicados"



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Aspergillus novoparasiticus: a new clinical species of the section Flavi -2011

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During a survey on the incidence of Aspergillus in clinical environments, we found some interesting isolates that were morphologically similar to Aspergillus parasiticus , but differed in the color of the colonies and in the pattern of their conidial ornamentation. In the present study, those isolates were characterized using a polyphasic approach. A phylogenetic analysis was carried out, based on partial...



Characteristics of 151 Brazilian Sporothrix schenckii Isolates from 5 Different Geographic Regions of Brazil: A Forgotten and Re-Emergent Pathogen – 2009

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Abstract: We used RAPD (random amplification of polymorphic DNA) to analyze the phenotypic and genotypic characteristics of Sporothrix schenckii isolated from five geographic regions of Brazil, from clinical, animal, and environmental sources. Our results yielded a significant difference (P<0.01) in the mean conidial area of S. schenckii animal isolates (2.96 ± 1.07) compared with those of clinical...



Treatment of subcutaneous phaeohyphomycosis and prospective follow-up of 17 kidney transplant recipients – 2009

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Background: Subcutaneous phaeohyphomycosis in solid organ recipients may have an adverse outcome. Objective: We sought to describe the disease course, treatment, and outcome of allograft function in kidney transplant recipients with phaeohyphomycosis. Methods: Seventeen patients were followed for a mean period of 25.4 months to analyze the clinical response to treatment. Results: There was no treatment...



Cerebral Aspergillosis Caused by Neosartorya hiratsukae, Brazil – 2002

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We report the first case of infection by Neosartorya hiratsukae, an ascomycete in which the conidial state resembles Aspergillus fumigatus. The fungus caused a brain infection in a Brazilian woman, who died despite itraconazole treatment. Diagnosis was established by direct microscopic examination, computed tomographic scan, and magnetic resonance imaging of the brain, and repeated cultures from the lesions....



Cross-transmission of vancomycin-resistant Enterococcus in patients undergoing dialysis and kidney transplant – 2010

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The objective of this study was to investigate the occurrence of vancomycin-resistant Enterococcus (VRE) cross-transmission between two patient groups (long-term dialysis and kidney transplant patients). Molecular typing, by automated ribotyping with the RiboPrinter Microbial Characterization System (Qualicon, USA), was used to analyze VRE isolates from 31 fecal samples of 320 dialysis patients and 38 fecal...



Identificación de Candida albicans utilizando el medio cromogénico Albicans ID – 2001

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La identificación correcta y precisa de los microorganismos es la base para la caracterización epidemiológica y tratamiento de las infecciones. El objetivo de nuestro estudio fue evaluar la eficiencia del medio cromogénico Albicans ID (bioMérieux, Francia) en la identificación de Candida albicans. De las 190 cepas evaluadas en el estudio, Candida albicans (80) y Candida dubliniensis (5) evidenciaron...



Genotyping of 44 Isolates of Fusarium solani, the Main Agent of Fungal Keratitis in Brazil – 2004

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In a retrospective study performed over 6 years in Brazil, Fusarium solani was found to be the most common species causing mycotic keratitis. The genetic diversity of 44 isolates from 39 patients was assessed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and PCR restriction fragment length polymorphism (PCR-RFLP) fingerprinting. ERIC-PCR was more discriminatory than PCR-RFLP for differentiating...



Evaluation of fluconazole resistance mechanisms in Candida albicans clinical isolates from HIV-infected patients in Brazil – 2004

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In this study, we describe resistance mechanisms in fluconazole-resistant isolates of C. albicans isolated from AIDS patients from nine Brazilian hospitals. These mechanisms include the presence of point mutations in the ERG11 gene and overexpression of ERG11, and several genes encoding efflux pumps, as measured by quantitative real-time reverse transcriptase polymerase chain reaction. Several fluconazole-resistant...



Estudo laboratorial das micoses oculares e fatores associados às ceratites – 2005

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Objetivo: Analisar a freqüência e a etiologia das doenças oculares micóticas diagnosticadas por cultura no Laboratório de Doenças Externas Oculares da Universidade Federal de São Paulo (LOFT-UNIFESP), São Paulo, SP, Brasil e os fatores associados às ceratites fúngicas. Métodos: Estudo retrospectivo de 14.391 solicitações de exames laboratoriais de doenças infecciosas oculares no período...



Estudo da micobiota em conjuntiva sadia de diabéticos, residentes na área urbana da cidade de São Paulo – Brasil – 2006

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Objetivos: Determinar a micobiota de conjuntiva sadia em indivíduos diabéticos, segundo tipo de diabetes, idade, sexo, tempo de doença, tipo de tratamento e estádio da retinopatia. Estabelecer a micobiota anemófila nas salas de colheita. Métodos: Estudo transversal de 803 diabéticos residentes na zona urbana de São Paulo - SP/Brasil. Foi usado para primoisolamento o meio de cultivo ágar Sabouraud...